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KMID : 0861120010050020106
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Korean Journal of Oriental Preventive Medicine
2001 Volume.5 No. 2 p.106 ~ p.113
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Cytotoxic Evaluation of the Ethyl Acetate Soluble Fraction of Perilla frutescens
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Kim Sung-Eun
Chun Hyun-Ja
Kim Il-Kwang
Han Du-Seok
Lee Hyun-Ok
Ahn Jong-Woong
Lee Mee-Hee
Baek Seung-Hwa
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Abstract
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The Cytotoxic activity of the ethyl acetate soluble fraction of Perilla frutescens on human oral epithelioid carcinoma cell lines was evaluated by 3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyl-2H-tetrazalium bromide (MTT) colorimetric method. The light microscopic study was carried out to observe the morphological changes of cultured human oral epitheloid carcinoma cell lines (KB) These results were obtained as follows; 1. subfraction 1 of the ethyl acetate soluble fraction of perilla frutescens was shown significant cytotoxic activity (p<0.001) at 10-2 mg/ml concentration, this concentration was decreased the cytotoxic activity of 70.3% . 2. The comparison of IC50 values of various subfractions in human oral epithelioid carcinoma cell lines was exhibited that their susceptibility to these subfractions decreased in the following order ; subfraction 5 > subfraction 4 > subfraction 3 > subfraction 2 > subfraction 1 by MTT assay. In light microscopy, the subfraction 1 of the ethyl acetate soluble fraction of Perilla frutescens showed the highest cytotoxic activity. These findings suggest that subfraction 1 possessed the most cytotoxic constituents.
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KEYWORD
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Perilla frutescens, MTT assay, Cytotoxic activity, Human oral epithelioid carcinoma cell
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